Anti-Interleukin-9 Antibody Increases the Effect of Allergen-Specific Immunotherapy in Murine Allergic Rhinitis

作者:Shin Ji Hyeon; Kim Do Hyun; Kim Boo Young; Kim Sung Won; Hwang Se Hwan; Lee Joohyung; Kim Soo Whan
来源:Allergy Asthma & Immunology Research, 2017, 9(3): 237-246.
DOI:10.4168/aair.2017.9.3.237

摘要

Purpose: Interleukin (IL)-9 induces allergic responses; however, the roles of anti-IL-9 antibody in the induction of tolerance remain unclear. This study investigated the effects of anti-IL-9 antibody on oral tolerance (OT) in a mouse model of allergic rhinitis (AR). Methods: BALB/c mice were divided into 4 groups: the control, AR, OT, and OT with anti-IL-9 antibody (0T+IL9AB) groups. Ovalbumin (OVA) was used for sensitization and challenge. Mice in the OT and 0T+IL9AB groups were fed OVA for immunotherapy. During immunotherapy, 0T+IL9AB mice were injected with anti-IL-9 antibody. Allergic symptoms, tissue eosinophil counts, and serum OVA-specific immunoglobulin E (IgE) were measured. The mRNA expressions of cytokines and transcription factors of T cells of nasal mucosa were determined by real-time polymerase chain reaction (PCR). The protein levels of GATA3, ROR-gamma t, and Foxp3 in nasal mucosa were determined by Western blot. CD4(+)CD25(+)Foxp3(+) T cells in the spleen were analyzed by flow cytometry. Results: Administration of anti-IL-9 antibody decreased allergic symptoms, OVA-specific IgE levels, and eosinophil counts. In addition, it inhibited T-helper (Th) 2 responses, but had no effect on Th1 responses. Protein levels of ROR-gamma t and mRNA levels of PU.1 and ROR-gamma t were reduced by anti-IL-9 antibody. Anti-IL-9 antibody increased Foxp3 and IL-10 mRNA expression, Foxp3 protein, and induction of CD4+CD25+Foxp3+ T cells. Conclusions: Anti-IL-9 antibody decreased allergic inflammation through suppression of Th2 and Th17 cells. Anti-IL-9 antibody enhanced the tolerogenic effects of regulatory T cells. These results suggest that anti-IL-9 antibody might represent a potential therapeutic agent for allergen immunotherapy in patients with uncontrolled allergic airway disease.

  • 出版日期2017-5