Development of 3-D poly(trimethylenecarbonate-co-epsilon-caprolactone)-block-poly(p-dioxano ne) scaffold for bone regeneration with high porosity using a wet electrospinning method

作者：Shin Teo Jeon; Park Sook Young; Kim Hyun Jeong^*; Lee Hak Jun; Youk Ji Ho

来源：Biotechnology Letters, 2010, 32(6): 877-882.

DOI：10.1007/s10529-010-0235-7

• Biomaterials
• Bone regeneration
• Cell movement
• Osteoblasts
• Poly(p-dioxanone)

The three dimensional (3-D) poly(trimethylenecarbonate-co-epsilon-caprolactone)-block-poly(p-dioxanone) scaffold was made using a wet electrospinning method and its application as a tissue engineered matrix was evaluated for bone regeneration. The scaffold was highly porous (90%) and interconnected among pores. Under scanning electron microscopy, the cells of the center of the scaffold showed healthy well attached shape even at 4 days after seeding. The osteoblastic MC3T3-E1 cells proliferated 1.2 times faster at 4 day, 1.5 times faster at 7 days after seeding as compared with the control in the scaffold (P < 0.05). The activity of alkaline phosphatase, a bone formation marker, of cells seeded in the scaffold was nearly four times faster compared to control 28 days after seeding (P < 0.05). Taken together, newly developed 3-D poly(trimethylenecarbonate-co-epsilon-caprolactone)-block-poly(p-dioxanone) scaffold is a promising candidate for bone regeneration.

• 出版日期2010-6