Antigen-Specific T-Regulatory Cells Can Extend Skin Graft Survival Time in Mice

作者：Yu, S.; Fu, B.; He, X.^*; Peng, X.; Hu, A.; Ma, Y.

来源：Transplantation Proceedings, 2011, 43(5): 2033-2040.

DOI：10.1016/j.transproceed.2011.02.063

• DENDRITIC CELLS
• EFFECTOR FUNCTION
• IN-VITRO
• EX-VIVO
• INDUCTION
• TOLERANCE
• EXPANSION
• BETA

This study investigated the effects of donor antigen-specific CD4(+)CD25(+) T-regulatory cells (Tregs) on skin allografts in mice. An allogeneic skin transplant model was established using donor C57BL/6 or DBA and recipient BALB/c mice. Recipients were divided into 4 groups: control group without intervention (CON; C57BL/6 to BALB/c), rapamycin gavage group (RAP; C57BL/6 to BALB/c), CD4(+)CD25(+) Tregs-treated group (TRE; C57BL/6 to BALB/c), in which recipients received transfusions of CD4(+)CD25(+) Tregs stimulated with C57BL/6-derived immature dendritic cells, and the third-party donor group (DBA; DBA to BALB/c) in which recipients received transfusions of BALB/c CD4(+)CD25(+) Tregs stimulated with C57BL/6-derived immature dendritic cells. Mean (SD) survival time of the skin allografts in the TRE group was 17.0 (3.4) days, significantly longer than in the other groups: CON, 6.9 (1.9) days; RAP, 10.3 (3.0) days; and DBA, 10.8 (3.6) days. The TRE group demonstrated a significantly greater expression of transforming growth factor-beta and interleukin (IL)-10. Donor antigen-specific CD4(+)CD25(+) Tregs effectively extend skin allograft survival in mice.

• 出版日期2011-6
• 单位佛山市第一人民医院; 中山大学; 昆明医科大学