Aptamer photoregulation in vivo

作者：Li Lele; Tong Rong; Chu Hunghao; Wang Weiping; Langer Robert^*; Kohane Daniel S

来源：Proceedings of the National Academy of Sciences of the United States of America, 2014, 111(48): 17099-17103.

DOI：10.1073/pnas.1420105111

• aptamer
• light triggering
• cancer targeting

The in vivo application of aptamers as therapeutics could be improved by enhancing target-specific accumulation while minimizing off-target uptake. We designed a light-triggered system that permits spatiotemporal regulation of aptamer activity in vitro and in vivo. Cell binding by the aptamer was prevented by hybridizing the aptamer to a photo-labile complementary oligonucleotide. Upon irradiation at the tumor site, the aptamer was liberated, leading to prolonged intratumoral retention. The relative distribution of the aptamer to the liver and kidney was also significantly decreased, compared to that of the free aptamer.

• 出版日期2014-12-2
• 单位MIT