A highly specific competitive direct enzyme immunoassay for sterigmatocystin as a tool for rapid immunochemotaxonomic differentiation of mycotoxigenic Aspergillus species

作者：Wegner S; Bauer J I; Dietrich R; Maertlbauer E; Usleber E; Gottschalk C; Gross M^*

来源：Letters in Applied Microbiology, 2017, 64(2): 124-130.

DOI：10.1111/lam.12702

• aflatoxin
• Aspergillus
• immunoassay
• mycotoxin
• polycolonal antibodies
• sterigmatocystin

A simplified method to produce specific polyclonal rabbit antibodies against sterigmatocystin (STC) was established, using a STC-glycolic acid-ether derivative (STC-GE) conjugated to keyhole limpet haemocyanin (immunogen). The competitive direct enzyme immunoassay (EIA) established for STC had a detection limit (20% binding inhibition) of 130 pg ml(-1). The test was highly specific for STC, with minor cross-reactivity with O-methylsterigmatocystin (OMSTC, 0.87%) and negligible reactivity with aflatoxins (< 0.02%). STC-EIA was used in combination with a previously developed specific EIA for aflatoxins (< 0.1% cross-reactivity with STC and OMSTC), to study the STC/aflatoxin production profiles of reference strains of Aspergillus species. This immunochemotaxonomic procedure was found to be a convenient tool to identify STC- or aflatoxin-producing strains.

• 出版日期2017-2