A secretome view of colonisation factors in Shiga toxin-encoding Escherichia coli (STEC): from enterohaemorrhagic E. coli (EHEC) to related enteropathotypes

作者：Monteiro Ricardo; Ageorges Valentin; Rojas Lopez Maricarmen; Schmidt Herbert; Weiss Agnes; Bertin Yolande; Forano Evelyne; Jubelin Gregory; Henderson Ian R; Livrelli Valerie; Gobert Alain P; Rosini Roberto; Soriani Marco; Desvaux Mickael

来源：FEMS Microbiology Letters, 2016, 363(16): fnw179.

DOI：10.1093/femsle/fnw179

• secretion systems
• cell-surface display
• surface organelle
• outer membrane proteins
• autotransporters
• bacterial protein secretion

Shiga toxin-encoding Escherichia coli (STEC) regroup strains that carry genes encoding Shiga toxin (Stx). Among intestinal pathogenic E. coli, enterohaemorrhagic E. coli (EHEC) constitute the major subgroup of virulent STEC. EHEC cause serious human disease such as haemorrhagic colitis and haemolytic-uremic syndrome. While EHEC have evolved from enteropathogenic E. coli, hybrids with enteroaggregative E. coli have recently emerged. Of note, some enteroinvasive E. coli also belong to the STEC group. While the LEE (locus of enterocyte effacement) is a key and prominent molecular determinant in the pathogenicity, neither all EHEC nor STEC contain the LEE, suggesting that they possess additional virulence and colonisation factors. Currently, nine protein secretion systems have been described in diderm-lipopolysaccharide bacteria (archetypal Gram-negative) and can be involved in the secretion of extracellular effectors, cell-surface proteins or assembly of cell-surface organelles, such as flagella or pili. In this review, we focus on the secretome of STEC and related enteropathotypes, which are relevant to the colonisation of biotic and abiotic surfaces. Considering the wealth of potential protein trafficking mechanisms, the different combinations of colonisation factors and modulation of their expression is further emphasised with regard to the ecophysiology of STEC.

• 出版日期2016-8