alpha-Tocopherol supplementation of allergic female mice inhibits development of CD11c(+)CD11b(+) dendritic cells in utero and allergic inflammation in neonates

作者：Abdala Valencia Hiam; Berdnikovs Sergejs; Soveg Frank W; Cook Mills Joan M^*

来源：American Journal of Physiology - Lung Cellular and Molecular Physiology, 2014, 307(6): L482-L496.

DOI：10.1152/ajplung.00132.2014

• allergic lung inflammation
• vitamin E

alpha-Tocopherol blocks responses to allergen challenge in allergic adult mice, but it is not known whether alpha-tocopherol regulates the development of allergic disease. Development of allergic disease often occurs early in life. In clinical studies and animal models, offspring of allergic mothers have increased responsiveness to allergen challenge. Therefore, we determined whether alpha-tocopherol blocked development of allergic responses in offspring of allergic female mice. Allergic female mice were supplemented with alpha-tocopherol starting at mating. The pups from allergic mothers developed allergic lung responses, whereas pups from saline-treated mothers did not respond to the allergen challenge, and alpha-tocopherol supplementation of allergic female mice resulted in a dose-dependent reduction in eosinophils in the pup bronchoalveolar lavage and lungs after allergen challenge. There was also a reduction in pup lung CD11b(+) dendritic cell subsets that are critical to development of allergic responses, but there was no change in several CD11b(+) dendritic cell subsets. Furthermore, maternal supplementation with alpha-tocopherol reduced the number of fetal liver CD11b(+) dendritic cells in utero. In the pups, there was reduced allergen-induced lung mRNA expression of IL-4, IL-33, TSLP, CCL11, and CCL24. Cross-fostering pups at the time of birth demonstrated that alpha-tocopherol had a regulatory function in utero. In conclusion, maternal supplementation with alpha-tocopherol reduced fetal development of subsets of dendritic cells that are critical for allergic responses and reduced development of allergic responses in pups from allergic mothers. These results have implications for supplementation of allergic mothers with alpha-tocopherol.

• 出版日期2014-9-15
• 单位西北大学