Detection of Target ssDNA Using a Microfabricated Hall Magnetometer with Correlated Optical Readout

作者:Hira Steven M; Aledealat Khaled; Chen Kan Sheng; Field Mark; Sullivan Gerard J; Chase P Bryant; Xiong Peng; von Molnar Stephan; Strouse Geoffrey F*
来源:Journal of Biomedicine and Biotechnology, 2012, 492730.
DOI:10.1155/2012/492730

摘要

Sensing biological agents at the genomic level, while enhancing the response time for biodetection over commonly used, optics-based techniques such as nucleic acid microarrays or enzyme-linked immunosorbent assays (ELISAs), is an important criterion for new biosensors. Here, we describe the successful detection of a 35-base, single-strand nucleic acid target by Hall-based magnetic transduction as a mimic for pathogenic DNA target detection. The detection platform has low background, large signal amplification following target binding and can discriminate a single, 350 nm superparamagnetic bead labeled with DNA. Detection of the target sequence was demonstrated at 364 pM (%26lt;2 target DNA strands per bead) target DNA in the presence of 36 mu M nontarget (noncomplementary) DNA (%26lt;10 ppm target DNA) using optical microscopy detection on a GaAs Hall mimic. The use of Hall magnetometers as magnetic transduction biosensors holds promise for multiplexing applications that can greatly improve point-of-care (POC) diagnostics and subsequent medical care.

  • 出版日期2012

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