A novel in situ silver/hyaluronan bio-nanocomposite fabrics for wound crossMark and chronic ulcer dressing: In vitro and in vivo evaluations

作者：Abdel Mohsen A M^*; Jancar J; Abdel Rahman R M; Vojtek L; Hyrsl P; Duskova M; Nejezchlebova H

来源：International Journal of Pharmaceutics, 2017, 520(1-2): 241-253.

DOI：10.1016/j.ijpharm.2017.02.003

• In-situ silver
• Hyaluronan
• Cytotoxicity
• Wound dressing

In-situ formed hyaluronan/silver (HA/Ag) nanoparticles (NPs) were used to prepare composite fibers/ fabrics for the first time. Different concentrations of silver nitrate (1, 2 mg/100 ml) were added at ambient temperature to sodium hyaluronate solution (40 mg/ml), then the pH was increased to 8 by adding sodium hydroxide. The in-situ formed HA/Ag-NPs were used to prepare fibers/nonwoven fabrics by wet dry -spinning technique (WDST). UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag-0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively. The resultant HA/Ag-NPs1 and HA/Ag-NPs2 composite showed uniformly dispersed throughout HA fiber/fabrics (SEM), an excellent distribution of Ag-NPs with 25 +/- 2, nm size (TEM, DLS) and acceptable mechanical properties. The XRD analysis showed that the in situ preparation of Ag-NPs increased the crystallinity of the resultant fabrics as well as the thermal stability. The antibacterial performance of medical HA/Ag-NPs fabrics was evaluated against gram negative bacteria E. coli K12, exhibiting significant bactericidal activity. The fibers did not show any cytotoxicity against human keratinocyte cell line (HaCaT). In-vivo animal tests indicated that the prepared wound dressing has strong healing efficacy (non-diabetics/diabetics rat model) compared to the plain HA fabrics and greatly accelerated the healing process. Based on our results, the new HA/AgNPs-2 mg nonwoven wound dressing fabrics can be used in treating wounds and chronic ulcers as well as cell carrier in different biological research and tissue engineering.

• 出版日期2017-3-30