An economic approach for construction of a multiplexed capillary electrophoresis system

作者：Liu Shaorong^*; Pu Qiaosheng; Gao Lin; Lu Joann

来源：Talanta, 2006, 70(3): 644-650.

DOI：10.1016/j.talanta.2006.01.025

• multiplexed capillary electrophoresis
• capillary array electrophoresis
• optic fiber UV detection
• protein separation
• high throughput protein analysis

We describe in this report an economic approach to construct a multiplexed capillary electrophoresis (CE) system with optic-fiber UV detection. To demonstrate the feasibility of this approach, a seven-lane CE setup is built. The system has a background noise of similar to 10(-5) absorbance unit, a limit of detection of 3-4 mu M for uracil at 254 nm, and a linear dynamic range of 2.5 orders of magnitude. The apparatus consists of three major components: a UV light source, a photodiode array (PDA) and optical module assembly, and a computer. Compared to the recently reported 96-lane capillary electrophoresis system with UV-vis absorbance detection, the cost of goods is reduced by more than 10-fold, and the noise level is improved by 2 to 3 folds. Parallel CE separations of a protein mixture have been performed on this apparatus, and reproducible and high resolution separation results have been obtained. Detailed construction and characterization of the system have also been discussed.

• 出版日期2006-10-15