Murine liver-resident group 1 innate lymphoid cells regulate optimal priming of anti-viral CD8(+) T cells

作者：Krueger Peter D; Narayanan Sowmya; Surette Fionna A; Brown Michael G; Sung Sun Sang J; Hahn Young S^*

来源：Journal of Leukocyte Biology, 2017, 101(1): 329-338.

DOI：10.1189/jlb.3A0516-225R

• immunity
• viruses
• natural killer cells
• T lymphocytes

The liver contains 2 transcriptionally distinct group 1 ILC subsets: CD49a(+)ILC1s and CD49b(+)NK cells. However, little is known about how group 1 ILCs contribute to hepatic immune responses. Therefore, we characterized murine liver-resident group 1 ILCs and found that CD49a(+)ILC1s express high levels of the inhibitory receptor NKG2A and localize near DCs in perivascular spaces surrounding the portal triads. Upon hepatic viral infection, NKG2A signaling in group 1 ILCs, especially in CD49a(+)ILC1s, inhibits CXCL9 expression required for robust accumulation of IFN-gamma(+)CD49b(+)NK cells. As a consequence, NKG2A(-/-) mice showed increased numbers of IFN-gamma-producing NK cells that preferentially activate liver CD103(+) DCs, leading to the sustained proliferation of adoptively transferred, virus-specific CD8(+) T cells. Collectively, these data suggest that group 1 ILCs play a role in maintaining the liver as a tolerogenic site by limiting the recruitment of peripheral NK cells during the early phase of viral infection. Furthermore, our findings implicate that the inhibition of NKG2A signaling on group 1 ILCs may be a novel vaccine strategy to induce robust CD8+ T cell responses against persistent liver pathogens.