Development and validation of a resistance and virulence gene microarray targeting Escherichia coli and Salmonella enterica

作者：Davis Margaret A^*; Lim Ji Youn; Soyer Yesim; Harbottle Heather; Chang Yung Fu; New Daniel; Orfe Lisa H; Besser Thomas E; Call Douglas R

来源：Journal of Microbiological Methods, 2010, 82(1): 36-41.

DOI：10.1016/j.mimet.2010.03.017

• Microarray
• Validation
• Resistance genes
• Enterobacteriaceae

A microarray was developed to simultaneously screen Escherichia coli and Salmonella enterica for multiple genetic traits. The final array included 203 60-mer oligonucleotide probes, including 117 for resistance genes, 16 for virulence genes, 25 for replicon markers, and 45 other markers. Validity of the array was tested by assessing inter-laboratory agreement among four collaborating groups using a blinded study design. Internal validation indicated that the assay was reliable (area under the receiver-operator characteristic curve = 0.97). Inter-laboratory agreement, however, was poor when estimated using the intraclass correlation coefficient, which ranged from 0.27 (95% confidence interval 0.24, 0.29) to 0.29 (0.23, 0.34). These findings suggest that extensive testing and procedure standardization will be needed before bacterial genotyping arrays can be readily shared between laboratories.

• 出版日期2010-7