Adhesion strength-based, label-free isolation of human pluripotent stem cells

作者：Singh Ankur; Suri Shalu; Lee Ted; Chilton Jamie M; Cooke Marissa T; Chen Weiqiang; Fu Jianping; Stice Steven L; Lu Hang; McDevitt Todd C; Garcia Andres J^*

来源：Nature Methods, 2013, 10(5): 438-+.

DOI：10.1038/NMETH.2437

We demonstrate substantial differences in 'adhesive signature' between human pluripotent stem cells (hPSCs), partially reprogrammed cells, somatic cells and hPSC-derived differentiated progeny. We exploited these differential adhesion strengths to rapidly (over similar to 10 min) and efficiently isolate fully reprogrammed induced hPSCs (hiPSCs) as intact colonies from heterogeneous reprogramming cultures and from differentiated progeny using microfluidics. hiPSCs were isolated label free, enriched to 95%-99% purity with >80% survival, and had normal transcriptional profiles, differentiation potential and karyotypes. We also applied this strategy to isolate hPSCs (hiPSCs and human embryonic stem cells) during routine culture and show that it may be extended to isolate hPSC-derived lineage-specific stem cells or differentiated cells.

• 出版日期2013-5