Anti-inflammatory and antinociceptive activities of Campomanesia adamantium

作者：Ferreira Leidiane C; Grabe Guimaraes Andrea; de Paula Carmen A; Michel Marcela C P; Guimaraes Raquel G; Rezende Simone A; de Souza Filho Jose D; Saude Guimaraes Denia A^*

来源：Journal of Ethnopharmacology, 2013, 145(1): 100-108.

DOI：10.1016/j.jep.2012.10.037

• Campomanesia adamantium
• Flavonols
• Anti-inflammatory
• Antinociceptive
• NO
• TNF-alpha
• IL-10

Ethnopharmacological relevance: Campomanesia species are used in folk medicine as anti-inflammatory, anti-rheumatic, anti-diarrheal and hypocholesterolemic. %26lt;br%26gt;Aim of the study: The present study investigated the in vivo anti-inflammatory and antinociceptive properties of ethyl acetate (AE) and aqueous (Aq) extracts from leaves of Campomanesia adamantium and in vitro anti-inflammatory activity of AE and its isolated flavonols, myricitrin and myricetin. %26lt;br%26gt;Materials and methods: The antinociceptive activity of AE and Aq was evaluated using acetic acid-induced writhing and formalin methods. The in vivo anti-inflammatory effect of AE and Aq was evaluated using carrageenan-induced paw oedema in mice. AE, myricitrin and myricetin were evaluated for their abilities to modulate the production of NO, TNF-alpha and IL-10 in LPS/IFN-gamma stimulated J774.A1 macrophages. %26lt;br%26gt;Results: It was found that orally administrated AE and Aq (125 and 250 mg/kg) inhibited carrageenan-induced paw oedema in mice. AE (125 and 250 mg/kg) and Aq (125 mg/kg) reduced the time to licking at the second phase of the formalin method in vivo in mice. AE (250 mg/kg) and Aq (125 mg/kg) also reduced the number of writhes. AE, myricitrin and myricetin inhibited NO (320 mu g/mL and 6.25-100 mu M, respectively) and TNF-alpha production by macrophages (320 mu g/mL for AE, 100 mu M for myricitrin and 25-100 mu M for myricetin). AE (160 and 320 mu g/mL), myricitrin (50 and 100 mu M) and myricetin (25-100 mu M) increased IL-10 production by macrophages. %26lt;br%26gt;Conclusions: The ethyl acetate and aqueous extracts from Campomanesia adamantium showed antinociceptive and anti-inflammatory effects supporting the use of the plant in folk medicine. The results suggest that anti-oedematogenic effect promoted by aqueous extract involves several anti-inflammatory mechanisms of action. The antinociceptive effect shown by aqueous extract can be due to the modulation of release of inflammatory mediators involved in nociception. The anti-inflammatory effects of AE and of its isolated flavonols may be attributed to inhibition of pro-inflammatory cytokines production, TNF-alpha and NO and to the increased of IL-10 production.

• 出版日期2013-1-9