Amperometric acetylcholinesterase biosensor based on multilayer multiwall carbon nanotubes- Chitosan composite

作者：Sun Xia^*; Zhai Chen; Wang Xiangyou

来源：Sensors and Transducers, 2011, 134(11): 182-191.

• Acetylcholinesterase
• Amperometric
• Carbofurans
• Chitosan composites
• Detection limits
• Detection sensitivity
• Electron-transfer reactions
• Enzymatic activities
• Fast response
• Glassy carbon electrodes
• Low detection limit
• Model compound
• Optimal conditions
• Pesticide analysis
• Quantitative determinations
• SIMPLE method
• Thiocholine

A simple method for immobilization of acetylcholinesterase (AChE) onto the glassy carbon electrode (GCE) modified with five layers of multiwall carbon nanotubes (MWNTs)-chitosan (CHIT) composite was proposed, and thus a fast, sensitive and stable amperometric sensor for quantitative determination of pesticides was developed. Five layers of MWNTs-CHIT promoted electron transfer reactions at a lower potential and catalyzed the electro-oxidation of thiocholine, thus, it improved the detection sensitivity of biosensor. Based on the inhibition of pesticides to the enzymatic activity of AChE, using carbofuran as a model compound, under optimal conditions, the inhibition of carbofuran was proportional to its concentration in two ranges, from 5×10-4 to 7.5 μg/mL and 7.5 to 20 μg/mL with a detection limit of 1×10-4 μg/mL. The constructed biosensor showed prominent characteristics and performances such as good precision, acceptable stability, fast response and low detection limit, which provided a new promising tool for pesticide analysis.

• 出版日期2011
• 单位山东大学