An introduction to DNA microarrays for gene expression analysis

作者：Karakach Tobias K; Flight Robert M; Douglas Susan E; Wentzell Peter D^*

来源：Chemometrics and Intelligent Laboratory Systems, 2010, 104(1): 28-52.

DOI：10.1016/j.chemolab.2010.04.003

• DNA microarray
• GeneChip
• Gene expression
• Experimental design

This tutorial presents a basic introduction to DNA microarrays as employed for gene expression analysis, approaching the subject from a chemometrics perspective. The emphasis is on describing the nature of the measurement process, from the platforms used to a few of the standard higher-level data analysis tools employed. Topics include experimental design, detection, image processing, measurement errors, ratio calculation, background correction, normalization, and higher-level data processing. The objective is to present the chemometrician with as clear a picture as possible of an evolving technology so that the strengths and limitations of DNA microarrays are appreciated. Although the focus is primarily on spotted, two-color microarrays, a significant discussion of single-channel, lithographic arrays is also included.

• 出版日期2010-11-15