Analysis of the functional activity of the 1.4-kb 5'-region of the rice actin 1 gene in stable transgenic plants of maize (Zea mays L)

作者：Zhong H; Zhang SB; Warkentin D; Sun BL; Wu TY; Wu R; Sticklen MB

来源：Plant Science, 1996, 116(1): 73-84.

DOI：10.1016/0168-9452(96)04369-5

• gene expression
• beta-glucuronidase (GUS)
• rice actin 1 gene (Actl)
• transgenic maize
• shoot meristem

The activity of the 5'-region of the rice actin 1 gene (Act1), covering a region 1,4 kb upstream of the Act1 translation initiation codon, was extensively analyzed in transgenic maize plants. The 5'-region of Act1 fused to the beta-glucuronidase (GUS) gene (gas) coding region was co-transformed to maize with the phosphinothricin acetyltransferase gene (bar) and the potato proteinase inhibitor II gene (pin2). One and 29 independent transformation events with expression of both bar and gus were recovered from bombardment of immature embryo-derived embryogenic callus of Hi-II derivative and bombardment of shoot tips of Honey N Pearl and Illinois Golden Extra Sweet, respectively. Expression of gus in tissues of transgenic plants was examined by histochemical assay, immunoblot analysis, and fluorometric GUS specific activity assay. A constitutive expression of the introduced gus was observed throughout the developmental stages of the vegetative and reproductive organs in transgenic maize plants. Quantitative analysis of GUS in transgenic plants showed that GUS, as percent of total soluble protein, was as much as 3.1% in leaves and 2.8% in roots, The functional activity of the 5'-region of Act1 was inherited to transgenic progeny. The results indicate that the 1.4-kb 5'-region of Act1 is an efficient and strong promoter for gene expression in stable transgenic maize plants.

• 出版日期1996-4-19