Gold-nanoparticle-modified polyvinylidene fluoride membranes used for western blotting with high sensitivity

作者：Tang Fengwu; Zhang Sai; Zhala Gahu; Huo Jingrui; Tu Yue; Xu Zhongwei; Fan Rong; Li Wei; Chen Xuyi; Chen Xiaoyi

来源：RSC Advances, 2014, 4(59): 31337-31340.

DOI：10.1039/c4ra05378h

In this study, we report an ultrasensitive western blotting method by introducing gold nanoparticles (AuNPs) onto modified polyvinylidene fluoride (PVDF) membranes via atom transfer radical polymerization (ATRP). AuNPs can prevent protein or peptide transfer across the PVDF membrane to reduce target loss. The results suggest that the improved western blotting method can be used to detect target biomarkers with a satisfactory limit of detection (LOD).

• 出版日期2014