Detection of Viruses By Counting Single Fluorescent Genetically Biotinylated Reporter Immunophage Using a Lateral Flow Assay

作者：Kim Jinsu; Adhikari Meena; Dhamane Sagar; Hagstroem Anna E V; Kourentzi Katerina; Strych Ulrich; Willson Richard C^*; Conrad Jacinta C

来源：ACS Applied Materials & Interfaces, 2015, 7(4): 2891-2898.

DOI：10.1021/am5082556

• bacteriophage
• diagnostics
• image processing
• immunoassay
• lateral-flow assay
• virus

We demonstrated a lateral flow immunoassay (LFA) for detection of viruses using fluorescently labeled M13 bacteriophage as reporters and single-reporter counting as the readout. AviTag-biotinylated M13 phage were functionalized with antibodies using avidinbiotin conjugation and fluorescently labeled with AlexaFluor 555. Individual phage bound to target viruses (here MS2 as a model) captured on an LFA membrane strip were imaged using epi-fluorescence microscopy. Using automated image processing, we counted the number of bound phage in micrographs as a function of target concentration. The resultant assay was more sensitive than enzyme-linked immunosorbent assays and traditional colloidal-gold nanoparticle LFAs for direct detection of viruses.

• 出版日期2015-2-4