Arteriogenesis. Genetic model for understanding the molecular mechanisms

作者：Troidl K^*; Jung G; Troidl C; Winterbauer C; Schaper W; Schmitz Rixen T

来源：Gefasschirurgie, 2012, 17(8): 727-730.

DOI：10.1007/s00772-012-1086-4

• Peripheral arterial occlusive disease
• Arteriogenesis
• Vascular remodeling
• Conditional knockout mice
• Smooth muscle cells

The remodeling of pre-existing arterioles into functional collateral vessels after occlusion of an artery is termed arteriogenesis. Understanding the molecular mechanisms of collateral growth provides an opportunity for therapeutic stimulation of this natural process and is the aim of arteriogenesis research. This beneficial vascular remodeling is associated with the proliferation of smooth muscle cells (SMC), which leads to an increase of the vascular lumen as well as wall thickness. In animal studies, a ligature of the femoral artery induces arteriogenesis. Subsequent tissue isolation of the collateral arteries allows molecular investigations in order to find the initial trigger of arteriogenesis. Differentially expressed genes, which have been identified in a genome-wide screening of growing collaterals, can be analyzed in terms of significance by targeted gene knock out. To further specify genetic modifications to the vascular wall, gene-targeting strategies that knock out target genes exclusively in a desired tissue while others remain unaffected have been established.

• 出版日期2012-12