Molecular mechanism of strigolactone perception by DWARF14

作者：Nakamura, Hidemitsu; Xue, You-Lin; Miyakawa, Takuya; Hou, Feng; Qin, Hui-Min; Fukui, Kosuke; Shi, Xuan; Ito, Emi; Ito, Shinsaku; Park, Seung-Hyun; Miyauchi, Yumiko; Asano, Atsuko; Totsuka, Naoya; Ueda, Takashi; Tanokura, Masaru; Asami, Tadao^*

来源：Nature Communications, 2013, 4(1): 2613.

DOI：10.1038/ncomms3613

• F-BOX PROTEIN
• ARABIDOPSIS-THALIANA
• PLANT HORMONE
• SIGNALING PATHWAYS
• CRYSTAL-STRUCTURES
• GIBBERELLIN
• RICE
• MAX2
• GERMINATION
• INHIBITION

Strigolactones (SLs) are phytohormones that inhibit shoot branching and function in the rhizospheric communication with symbiotic fungi and parasitic weeds. An alpha/beta-hydrolase protein, DWARF14 (D14), has been recognized to be an essential component of plant SL signalling, although its precise function remains unknown. Here we present the SL-dependent interaction of D14 with a gibberellin signalling repressor SLR1 and a possible mechanism of phytohormone perception in D14-mediated SL signalling. D14 functions as a cleavage enzyme of SLs, and the cleavage reaction induces the interaction with SLR1. The crystal structure of D14 shows that 5-hydroxy-3-methylbutenolide (D-OH), which is a reaction product of SLs, is trapped in the catalytic cavity of D14 to form an altered surface. The D14 residues recognizing D-OH are critical for the SL-dependent D14 - SLR1 interaction. These results provide new insight into crosstalk between gibberellin and SL signalling pathways.

• 出版日期2013-10