科研之友
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摘要
A manual non-radioactive DNA sequencing protocol was developed for rapid analysis of variable HTV-1 genomes. Sets of up to ten primers were used in one sequencing reaction. After polyacrylamide gel electrophoresis and blotting onto nylon membranes the individual sequences were detected by hybridization with digoxigenin-labelled oligonucleotides and chemiluminescence. The method is applicable to any sequencing project where numerous variants of DNA fragments of several 1000 bp of length are to be analysed.
- 出版日期1995-2
- 单位河北医科大学
