Bisphenol A (BPA) and atrazine inhibit the embryonic development of Ciona intestinalis (Ascidiacea, Urochordata)

作者：Cangialosi Maria Vittoria^*; Mansueto Valentina; Faqi Ali S

来源：Caryologia, 2013, 66(2): 97-102.

DOI：10.1080/00087114.2013.780438

• atrazine
• Ciona intestinalis
• bisphenol A
• developmental toxicity
• ecotoxicology
• evolution

The toxicities of bisphenol A (BPA) and atrazine in Ciona intestinalis development were examined in two separate series of experiments. The fertilized eggs were exposed to BPA or atrazine at concentrations of 0.1, 1 and 10 mu M. Pasteurized filtered sea water (PFSW) and lipid solvent (ethanol 0.0005%) solutions were used in the control groups. The embryos of PFSW and lipid solvent groups developed up to swimming larvae after 22 h post-fertilization. Both BPA and atrazine induced concentration-dependent effects on Ciona embryo development. In BPA 1 and 10 mu M groups the fertilized eggs were blocked at morula and at 2-4-cell stages, respectively. Changes noted in these groups include abnormal distribution of blastomeres that resulted in fused or absent blastomeres and lack of follicular and test cells. In all atrazine treated groups the development of fertilized eggs was blocked at 2-4 cells; no further development was observed beyond this stage. Anomalies noted include the unsegmented fertilized eggs and the fusion of blastomeres. The lowest observed effect concentration in BPA and atrazine was 1 and 0.1 mu M, with embryos blocked at morula and at 2-4-cell stage, respectively.

• 出版日期2013