Tomato bushy stunt virus (TBSV), a versatile platform for polyvalent display of antigenic epitopes and vaccine design

作者：Kumar Shantanu; Ochoa Wendy; Singh Pratik; Hsu Catherine; Schneemann Anette; Manchester Marianne; Olson Mark; Reddy Vijay^*

来源：Virology, 2009, 388(1): 185-190.

DOI：10.1016/j.virol.2009.02.051

• Virus-like particles
• Tomato bushy stunt virus
• Capsid display
• Vaccine design
• Ricin vaccine

Viruses-like particles (VLPs) are frequently being used as platforms for polyvalent display of foreign epitopes of interest oil their capsid Surface to improve their presentation enhancing the antigenicity and host immune response. In the present study, we used the VLPs of Tomato bushy Stunt Virus (TBSV). all icosahedral plant virus, as a platform to display 180 copies of 16 amino acid epitopes of ricin toxin fused to the C-terminal end of a modified TBSV capsid protein (N Delta 52). Expression of the chimeric recombinant protein in insect cells resulted in spontaneous assembly of VLPs displaying the ricin epitope. Cryo-electron microscopy and image reconstruction of the chimeric VLPs at 22 (A) over cap resolution revealed the locations all(] orientation of the ricin epitope exposed on the TBSV capsid Surface. Furthermore, injection of chimeric VI-l's into mice generated antisera that detected the native ricin toxin. The ease of fusing of short peptides of 15-20 residues and their ability to form two kinds (T=1, T=3) of bio-nanoparticles that result in the display of 60 or 180 copies of less constrained and highly exposed antigenic epitopes makes TBSV all attractive and versatile display platform for vaccine design.

• 出版日期2009-5-25