A Microfluidic Chip for ICPMS Sample Introduction

作者：Verboket Pascal E; Borovinskaya Olga; Meyer Nicole; Guenther Detlef; Dittrich Petra S

来源：Jove-Journal of Visualized Experiments, 2015, (97): e52525.

DOI：10.3791/52525

• Bioengineering
• Issue 97
• mass spectrometry
• ICPMS
• microfluidics
• droplet microfluidics
• monodisperse
• sample introduction
• chip
• red blood cells
• erythrocytes
• single cell analysis

This protocol discusses the fabrication and usage of a disposable low cost microfluidic chip as sample introduction system for inductively coupled plasma mass spectrometry (ICPMS). The chip produces monodisperse aqueous sample droplets in perfluorohexane (PFH). Size and frequency of the aqueous droplets can be varied in the range of 40 to 60 pm and from 90 to 7,000 Hz, respectively. The droplets are ejected from the chip with a second flow of PFH and remain intact during the ejection. A custom-built desolvation system removes the PFH and transports the droplets into the ICPMS. Here, very stable signals with a narrow intensity distribution can be measured, showing the monodispersity of the droplets. We show that the introduction system can be used to quantitatively determine iron in single bovine red blood cells. In the future, the capabilities of the introduction device can easily be extended by the integration of additional microfluidic modules.

• 出版日期2015-3