Amphritea ceti sp nova, isolated from faeces of Beluga whale (Delphinapterus leucas)

作者：Kim Young Ok; Park Sooyeon; Kim Doo Nam; Nam Bo Hye; Won Sung Min; An Du Hae; Yoon Jung Hoon^*

来源：International Journal of Systematic and Evolutionary Microbiology, 2014, 64(Pt 12): 4068-4072.

DOI：10.1099/ijs.0.067405-0

A Gram-stain-negative, aerobic, non-spore-forming, non-flagellated and rod-shaped or ovoid bacterial strain, designated RA1(T), was isolated from faeces collected from Beluga whale (Delphinapterus leucas) in Yeosu aquarium, South Korea. Strain RA1(T) grew optimally at 25 degrees C, at pH 7.0-8.0 and in the presence of 2.0% (w/v) NaCI. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain RA1(T) joins the cluster comprising the type strains of three species of the genus Amphritea, with which it exhibited 95.8-96.0% sequence similarity. Sequence similarities to the type strains of other recognized species were less than 94.3 %. Strain RA1(T) contained Q-8 as the predominant ubiquinone and summed feature 3 (C-16:1 omega 7c and/or C-16:1 omega 6c), C-18:1 omega 7c and C-16:0 as the major fatty acids. The major polar lipids of strain RA1(T) were phosphatidylethanolamine, phosphatidylglycerol, two unidentified lipids and one unidentified aminolipid. The DNA G+C content of strain RA1(T) was 47.4 mol%. The differential phenotypic properties, together with the phylogenetic distinctiveness, revealed that strain RA1(T) is separated from other species of the genus Amphritea. On the basis of the data presented, strain RA1(T) is considered to represent a novel species of the genus Amphritea, for which the name Amphritea ceti sp. nov. is proposed. The type strain is RA1 (=KCTC 42154(T)=NBRC 110551(T)).

• 出版日期2014-12