Determination of C-Terminal delta-Catenin Responsible for Inducing Dendritic Morphogenesis

作者：Lee Ho Bin; He Yongfeng; Yang Gyeong Su; Oh Jin A; Ha Ji Seon; Song Oh Hyuen; Lee Do Jin; Jung Sang Chul; Kim Kyung Keun; Kim Kwonseop; Kim Hangun^*

来源：Journal of Nanoscience and Nanotechnology, 2015, 15(8): 5589-5592.

DOI：10.1166/jnn.2015.10460

• delta-Catenin/NPRAP
• Dendrogenesis

delta-Catenin induces dendritic morphogenesis in several cells and it was reported that deletion of C-terminal 207 amino acid of delta-catenin completely abolished the dendritic morphogenesis. However, exact domain responsible for inducing dendritic morphogenesis in C-terminus of delta-catenin was not mapped. Here, we report that expression of Delta C47 (lacking 47 amino acid of C-terminus: 1-1200), Delta C77 (lacking 77 amino acid of C-terminus: 1-1170) deletion mutants of delta-catenin induced the dendritic morphogenesis of HEK293T and NIH3T3 cells as full-length delta-catenin did. In agreement with previous report, Delta C207 deletion mutant did not show the dendritic morphogenesis of the cells. Interestingly, introducing 107 amino acid deletion of C-terminus (Delta C107 mutant: 1-1140) and 177 amino acid deletion of C-terminus (Delta C177 mutant: 1-1070) showed limited primary and secondary dendritic process and notable spine-like process formation. These results suggest that 1140-1170 amino acid of C-terminal delta-catenin is required for primary and secondary dendrite-like process formation.

• 出版日期2015-8