An HPLC-UV Method for the Quantification of Zidovudine in Rat Plasma

作者：Carvalho Flavia Chiva^*; Campos Michel Leandro; Peccinini Rosangela Goncalves; Daflon Gremiao Maria Palmira

来源：Current Pharmaceutical Analysis, 2013, 9(4): 340-346.

• Liquid-liquid extraction
• pharmacokinetics
• rat plasma
• HPLC-UV bioanalytical methodology
• validation
• zidovudine

This work aims to develop and validate a simple methodology to quantify the most used antiretroviral, zidovudine (AZT), in rat plasma for preclinical studies. Some assays have been previously reported to quantify AZT in plasma; however, the majority of these methods uses complicated extraction methods. This method uses only 100 mu L plasma samples, which were precipitated with 100 mu L acidified acetonitrile containing an internal standard (IS). The plasma extracts were injected directly in a chromatographic system consisting of a UV-Vis detector (set at 266 nm) and an RP-C18 column. The mobile phase was an isocratic mixture of acetonitrile, methanol and 0.01% v/v aqueous formic acid (20: 20: 60 v/v/v) at a flow rate of 0.8 mL/min. Intra- and inter-day assay precision and accuracy variability were lower than 20% for the limit of quantification and 15% for higher concentrations. The applicability of the method was demonstrated by an in vivo study performed in rats. They were treated orally with an AZT-containing syrup and intravenously with an AZT solution. The calculated bioavailability of the syrup (56.7%) was in accordance with the literature. Therefore, this method can be used as an alternative to quantify plasma AZT in preclinical studies.

• 出版日期2013-11