Determination of brompheniramine enantiomers in rat plasma by cation-selective exhaustive injection and sweeping cyclodextrin modified electrokinetic chromatography method

作者：Yao, Yaqi; Zhao, Yu; Zhang, Xue; Yu, Jia^*; Guo, Xingjie^*

来源：Electrophoresis, 2018, 39(16): 2099-2106.

DOI：10.1002/elps.201800138

• Brompheniramine enantiomers
• CSEI-Sweeping-CDEKC
• Enantioseparation
• Stereoselective pharmacokinetic study

A method consisting of cation-selective exhaustive injection and sweeping (CSEI-sweeping) as online preconcentration followed by a cyclodextrin modified electrokinetic chromatography (CDEKC) enantioseparation has been developed for the simultaneous determination of two brompheniramine enantiomers in rat plasma. In this method, analytes were electrokinetically injected at a voltage of 8 kV for 80 s in a fused-silica capillary. Prior to the injection, the capillary was rinsed with 50mM phosphate buffer of pH 3.5, followed by a plug of a higher conductivity buffer (150mM phosphate pH 3.5, 20psi, 6min) and a plug of water (0.5psi, 5 s). Separation was carried out applying -20 kV in 50mM phosphate buffer, pH 3.5, containing 10% v/v ACN and 30mg/mL sulfated--cyclodextrin (S--CD). Analytical signals were monitored at 210 nm. The detection sensitivity of brompheniramine enantiomers was enhanced by about 2400-fold compared to the normal injection mode (hydrodynamic injection for 3 s at 0.5psi, with a BGE of 50mM phosphate buffer containing 20mg/mL S--CD at pH 3.5), and LLOQ of two enantiomers were both 0.0100g/mL. In addition, this method had fairly good repeatability and showed promising capabilities in the application of stereoselective pharmacokinetic investigations for brompheniramine enantiomers in rat.

• 出版日期2018-8
• 单位沈阳药科大学