Determination of thailandepsin B in rat plasma with a LC-MS/MS method

作者：Cheng Zhongzhe; Ding Lian; Zhang Yang; Chen Guiying; Chen Chang; Cheng Yi Qiang; Jiang Hongliang^*

来源：Bioanalysis, 2015, 7(1): 39-52.

DOI：10.4155/bio.14.264

Background: Thailandepsin B (TDP-B) is a potent histone deacetylase inhibitor under development. A reliable bioanalytical method for the quantification of TDP-B in plasma samples is required. Results: The stabilizer mixture containing hydrochloric acid, formic acid and dichlorvos was applied to stabilize TDP-B in matrix samples. The method validation was conducted over the curve range of 1.00 to 1000 ng/ml. The intrabatch and interbatch precision and accuracy of the quality control samples showed 10.9% relative standard deviation and -6.7% to 15.0% relative error. Moreover, a possible metabolite M of TDP-B was tentatively characterized. Conclusion: A reliable LC-MS/MS method was developed and validated for the quantification of TDP-B and was successfully applied to a rat pharmacokinetic study.

• 出版日期2015
• 单位华中科技大学