Medium-Pressure Liquid Chromatography Coupled to Electrospray Ionization Mass Spectrometry for Separation and On-Line Characterization of Flavonoids from Asparagus officinalis

作者：Hou Zhiguo; Luo Jianguang; Kong Lingyi^*

来源：Chromatographia, 2009, 70(9-10): 1447-1450.

DOI：10.1365/s10337-009-1329-z

• Column liquid chromotography
• Medium-pressure liquid chromatography
• Mass spectrometry
• Flavonoids
• Asparagus officinalis

A novel method for separation and on-line characterization of flavonoids from Asparagus officinalis by medium-pressure liquid chromatography coupled to electrospray ionization multi-stage mass spectrometry (MPLC-ESI-MS(n)) was successfully established. The hyphenation between MPLC and ESI-MS(n) was designed to keep the split ratio exactly in the range from 1:100 to 1:300. The separation procedure was guided by the chromatogram of ion current of MS(n) and the structures of compounds were characterized by fragments information at the same time. Consequently, it was proved that MPLC coupled with ESI-MS(n) was an effective method for separation of compounds from multi-component mixtures with high purity and desired amounts and simultaneous elucidation of chemical structures.

• 出版日期2009-11
• 单位中国药科大学