Mitochondrial membrane potential decrease caused by loss of PINK1 is not due to proton leak, but to respiratory chain defects

作者：Amo Taku; Sato Shigeto; Saiki Shinji; Wolf Alexander M; Toyomizu Masaaki; Gautier Clement A; Shen Jie; Ohta Shigeo; Hattori Nobutaka^*

来源：Neurobiology of Disease, 2011, 41(1): 111-118.

DOI：10.1016/j.nbd.2010.08.027

• Parkinson's disease
• Mitochondria
• PINK 1
• Parkin
• Membrane potential
• Oxidative phosphorylation
• Modular kinetic analysis
• Proton leak
• Reactive oxygen species

Mutations in PTEN-induced putative kinase 1 (PINK1) cause a recessive format Parkinson's disease (PD). PINK1 is associated with mitochondrial quality control and its partial knock-down induces mitochondrial dysfunction including decreased membrane potential and increased vulnerability against mitochondrial toxins, but the exact function of PINK1 in mitochondria has not been investigated using cells with null expression of PINK1. Here, we show that loss of PINK1 caused mitochondria! dysfunction. In PINK1-deficient (PINK1(-/-)) mouse embryonic fibroblasts (MEFs), mitochondrial membrane potential and cellular ATP levels were decreased compared with those in littermate wild-type MEFs. However, mitochondrial proton leak, which reduces membrane potential in the absence of ATP synthesis, was not altered by loss of PINK1. Instead, activity of the respiratory chain, which produces the membrane potential by oxidizing substrates using oxygen, declined. H(2)O(2) production rate by PINK1(-/-) mitochondria was lower than PINK1(+/+) mitochondria as a consequence of decreased oxygen consumption rate, while the proportion (H(2)O(2) production rate per oxygen consumption rate) was higher. These results suggest that mitochondrial dysfunctions in PD pathogenesis are caused not by proton leak, but by respiratory chain defects.

• 出版日期2011-1