A simple but effective fluorescent probe with large stokes shift for specific detection of cysteine in living cells

作者：Chen, Song^*; Hou, Peng; Wang, Jing; Fu, Shuang; Liu, Lei

来源：Journal of Photochemistry and Photobiology A: Chemistry , 2018, 363: 7-12.

DOI：10.1016/j.jphotochem.2018.05.025

• Stokes shift
• Fluorescent probe
• Cell imaging
• Cystein

A novel 4'-hydroxybiphenyl-4-carbonitrile-based fluorescent probe, 1, for selective detection of cystein (Cys) over homocystein (Hcy) and glutathione (GSH) was developed. This probe had simple structure and could be easily synthesized with good yield from commercially available materials. Moreover, probe 1 showed a remarkable large Stokes shift (180 nm) and displayed a rapid (5 min) and highly sensitive response (the detection limit was 0.15 mu M) for Cys with fluorescence turn-on signal changes (142-fold fluorescence enhancement). Importantly, probe 1 could be used to detect and image both exogenous and endogenous Cys in living A549 cells.

• 出版日期2018-8-1
• 单位齐齐哈尔医学院