A Green Route Towards Highly Photoluminescent and Cytocompatible Carbon dot Synthesis and its Separation Using Sucrose Density Gradient Centrifugation

作者：Oza, Goldie^*; Oza, Kusum; Pandey, Sunil; Shinde, Sachin; Mewada, Ashmi; Thakur, Mukeshchand; Sharon, Maheshwar; Sharon, Madhuri

来源：Journal of Fluorescence, 2015, 25(1): 9-14.

DOI：10.1007/s10895-014-1477-x

• Carbon nanostructures
• Chemical synthesis
• X-ray diffraction
• Luminescence

An efficient, fast and green method for synthesis of Carbon dots (C-dots) using natural precursor Citrus limone under ultrasonic condition is demonstrated. Such as-synthesized C-dots were further purified using Sucrose density gradient centrifugation method (SDGC) which resulted in the separation of water-soluble, photo luminescent, monodispersed, highly photostable and chemically stable C-dot fractions (F1 and F2). They possess very small size (5-20 nm) as evidenced by High angle annular dark field-Scanning Transmission electron microscopy (HAADF-STEM) and very strong luminescence as shown by fluorescence spectroscopic studies. Cytocompatibility and bio imaging properties of both the fractions (F1 and F2) were then studied on Hep-2 cells. Quantum yield of F1 and F2 fraction was found to be 12.1 and 15 %, respectively.

• 出版日期2015-1
• 单位中山大学