Allelic variations of LcyE and crtRB1 genes in tropical adapted yellow endosperm maize inbred lines

作者：Adeyemo Oyenike^*; Omidiji Olusesan

来源：Indian Journal of Genetics and Plant Breeding, 2013, 73(4): 363-370.

DOI：10.5958/j.0975-6906.73.4.055

• Maize inbred lines
• allele specific markers
• beta-carotene
• pro-vitamin A

Maize is an important staple cereal crop in sub-Saharan Africa. Tropical-adapted yellow endosperm maize varieties in Africa have low amounts of pro-vitamin A. Use of molecular markers that detect alleles associated with high level of pro-vitamin A can help to improve the pro-vitamin A content in maize through conventional breeding. In this study polymerase chain reaction (PCR) based markers were used to characterize two sets of tropical-adapted yellow endosperm maize inbred lines for allelic variants, present in two carotenoid biosynthesis genes encoding lycopene epsilon cyclase (LcyE) and beta-carotene hydroxylase 1 (crtRB1). Frequency of the favourable allele at LcyE 3'TE polymorphism is 29% in Set 1 and 67% in Set 2, and the 5'TE was present at frequencies of 24% in Si and 3.3% in 2. The frequency of the favourable allele at the LcyESNP216 polymorphism was more than 95% in both the sets of inbred lines. None of the lines included in Set 1 had favourable alleles for crtRB1. On the other hand, in Set 2 the favourable allele in the crtRB15'TE insertion, had a frequency of 14% whereas the favourable allele-frequency was 11% for crtRB1 3'TE and was 1% for InDel4. Twelve maize inbred lines (9.8%) had favourable alleles for crtRB1 3'TE and 5'TE in Set 2. None of the maize inbred lines carried favourable alleles for all three crtRB1 polymorphisms. Favourable haplotypes of LcyE and crtRB1 were found together in fifteen (12%) inbred lines.

• 出版日期2013-11