A LACTOCOCCUS-LACTIS GENE ENCODES A MEMBRANE-PROTEIN WITH PUTATIVE ATPASE ACTIVITY THAT IS HOMOLOGOUS TO THE ESSENTIAL ESCHERICHIA-COLI FTSH GENE-PRODUCT

作者：NILSSON D^*; LAURIDSEN AA; TOMOYASU T; OGURA T

来源：Microbiology-SGM, 1994, 140: 2601-2610.

• LACTOCOCCUS LACTIS
• FTSH
• (TMA)
• MEMBRANE PROTEIN
• AAA-PROTEIN FAMILY

A gene, encoding a protein homologous to an essential Escherichia coli protein, FtsH. was identified adjacent to the hpt gene and the tmA operon in the Gram-positive bacterium Lactococcus lactis. The deduced amino acid sequence of the gene product showed full-length similarity to FtsH of E. coli, Yme1p of Saccharomyces cerevisiae and a conserved region found in a new family of putative ATPases. In-frame fusions of L. lactis ftsH and phoA1 in E. coli, and immunodetection of the L. lactis FtsH protein in cell fractions using University School of anti-e coli FtsH serum showed that L. lactis ftsH was expressed and encodes a membrane protein. When contained on a high copy number plasmid, the L. lactis ftsH gene complemented the lethality of a Delta ftsH3::kan mutation in E. coli at 37 degrees C and below, indicating that the L. lactis ftsH gene can functionally replace the E. coli ftsH gene to some extent. The resulting E. coli strain showed temperature sensitivity and salt sensitivity. A L. lactis mutant with an insertion into ftsH was salt-, heat- and cold-sensitive. These results suggest that FtsH is somehow involved in stress responses. Southern hybridization analysis indicated that genes homologous to ftsH of L. lactis were also present in Bacillus subtilis, and several Lactobacillus and Leuconostoc species, suggesting high conservation of ftsH in bacterial species.

• 出版日期1994-10