Analysis of human plasma lipids by using comprehensive two-dimensional gas chromatography with dual detection and with the support of high-resolution time-of-flight mass spectrometry for structural elucidation

作者：Salivo Simona; Beccaria Marco; Sullini Giuseppe; Tranchida Peter Q; Dugo Paola; Mondello Luigi^*

来源：Journal of Separation Science, 2015, 38(2): 267-275.

DOI：10.1002/jssc.201400844

• Comprehensive two-dimensional gas chromatography
• High-resolution time-of-flight mass spectrometry
• Human plasma
• Lipidomics
• Triacylglycerols

The main focus of the present research is the analysis of the unsaponifiable lipid fraction of human plasma by using data derived from comprehensive two-dimensional gas chromatography with dual quadrupole mass spectrometry and flame ionization detection. This approach enabled us to attain both mass spectral information and analyte percentage data. Furthermore, gas chromatography coupled with high-resolution time-of-flight mass spectrometry was used to increase the reliability of identification of several unsaponifiable lipid constituents. The synergism between both the high-resolution gas chromatography and mass spectrometry processes enabled us to attain a more in-depth knowledge of the unsaponifiable fraction of human plasma. Additionally, information was attained on the fatty acid and triacylglycerol composition of the plasma samples, subjected to investigation by using comprehensive two-dimensional gas chromatography with dual quadrupole mass spectrometry and flame ionization detection and high-performance liquid chromatography with atmospheric pressure chemical ionization quadrupole mass spectrometry, respectively.

• 出版日期2015-1