A rapid high throughput proteomic method based on profiling of proteolytic free peptides to assess post-delivery degradation of placental tissue

作者：Heywood Wendy E^*; Pryce Jeremy; Virasami Alex; Preece Rhian Lauren; Dezateux Carol; Mills Kevin; Sebire Neil J

来源：Placenta, 2016, 44: 109-111.

DOI：10.1016/j.placenta.2016.04.014

• Placenta
• Proteolysis
• Multivariate analysis
• LC-MS
• Free peptide

A rapid method to determine quality for placental proteomic studies is required due to varying lengths of time between delivery and sampling in routine protocols. We developed a rapid 10 min LC-MS based scanning method to profile free peptides liberated from natural proteolytic degradation. The assay was applied to placenta samples obtained following refrigeration for varying time periods post-delivery (12 h, +24 h, +48 h and +72 h). Analysis reveals time dependant overlapping profiles for groups <24 to +48 h with greatest variation in the +72 h group, indicating that significant proteolysis affects tissue integrity between 48 and 72 h.

• 出版日期2016-8