A novel biosensor based on boronic acid functionalized metal-organic frameworks for the determination of hydrogen peroxide released from living cells

作者：Dai, Hongxia; Lu, Wenjuan^*; Zuo, Xianwei; Zhu, Qian; Pan, Congjie; Niu, Xiaoying; Liu, Juanjuan; Chen, HongLi; Chen, Xingguo^*

来源：Biosensors and Bioelectronics, 2017, 95: 131-137.

DOI：10.1016/j.bios.2017.04.021

• Metal-organic frameworks
• Boronic acid
• Hydrogen peroxide
• Horseradish peroxidase
• Electrochemical biosensor

In this work, we report a durable and sensitive 11202 biosensor based on boronic acid functionalized metal organic frameworks (denoted as MIL-100(Cr)-B) as an efficient immobilization matrix of horseradish peroxidase (HRP). MIL-100(Cr)-B features a hierarchical porous structure, extremely high surface area, and sufficient recognition sites, which can significantly increase HRP loading and prevent them from leakage and deactivation. The H2O2 biosensor can be easily achieved without any complex processing. Meanwhile, the immobilized HRP exhibited enhanced stability and remarkable catalytic activity towards H2O2 reduction. Under optimal conditions, the biosensor showed a fast response time (less than 4 s) to H2O2 in a wide linear range of 0.5-3000 mu M with a low detection limit of 0.1 mu M, as well as good anti-interference ability and long-term storage stability. These excellent performances substantially enable the proposed biosensor to be used for the real-time detection of H2O2 released from living cells with satisfactory results, thus showing the potential application in the study of H2O2-involved dynamic pathological and physiological process.

• 出版日期2017-9-15
• 单位功能有机分子化学国家重点实验室; 兰州大学