Dietary Vitamin D during Pregnancy Has Dose-Dependent Effects on Long Bone Density and Architecture in Guinea Pig Offspring but Not the Sows

作者：Tabatabaei Negar; Rodd Celia J; Kremer Richard; Khavandgar Zohreh; Murshed Monzur; Weiler Hope A^*

来源：Journal of Nutrition, 2014, 144(12): 1985-1993.

DOI：10.3945/jn.114.197806

• vitamin D
• dose-response
• pregnancy
• bone
• guinea pig

Background: The effects of vitamin D during pregnancy on maternal and neonatal bone health remain unclear. %26lt;br%26gt;Objective: This study was designed to test whether dietary vitamin D dose-dependently affects maternal and neonatal bone health. %26lt;br%26gt;Methods: Female guinea pigs (n = 45; 4 mo old) were randomly assigned at mating to receive 1 of 5 doses of vitamin D-3 (cholecalciferol; 0, 0.25, 0.5, 1, or 2 IU/g diet) throughout pregnancy. Plasma vitamin D metabolites, mineral homeostasis, bone biomarkers, and bone mass were tested in sows throughout pregnancy and in 2-d-old pups. Microarchitecture and histology of excised bone were conducted postpartum. %26lt;br%26gt;Results: By 3 wk of pregnancy, plasma 25-hydroxyvitamin D (25(OH)D) followed a positive dose-response, whereas 1,25-dihydroxyvitamin D[1,25(OH)(2)D] reached a plateau if vitamin D was %26gt;= 0.5 IU/g diet. Weight gain, areal bone mineral density (aBMD), volumetic bone mineral density (vBMD), and bone biomarkers did not differ among maternal groups. A positive dose-response was observed for mean +/- SEM pup plasma concentrations of 25(OH)D (10.5 +/- 1.50 to 113 +/- 11.6 nmol/L) and 1,25(OH)(2)D (123 +/- 13.8 to 544 +/- 53.3 pmol/L). Pup weight, plasma minerals, and osteocalcin were not different; plasma deoxypyridinoline was lower in the 1- and 0.25-IU/g groups than in all other groups. Pup femur aBMD was higher 19.2-13%; P = 0.04) in the 2-IU/g group than in all other groups except for the 0-IU/g group. Tibia and femur vBMD of pups responded to maternal diet in a U-shaped pattern. The femoral growth plate was 7.9% wider in the 0-IU/g group than in the 1-IU/g group. %26lt;br%26gt;Conclusions: Maternal vitamin D supplementation dose-dependently altered pup long bone architecture and mineral density in a manner similar to vitamin D deficient rickets whereas maternal bone was stable. These data reinforce that inadequate maternal vitamin D intake may compromise neonatal bone health and that exceeding recommendations is not advantageous.

• 出版日期2014-12
• 单位McGill