Aptasensor for electrochemical sensing of angiogenin based on electrode modified by cationic polyelectrolyte-functionalized graphene/gold nanoparticles composites

作者：Chen, Zhengbo^*; Zhang, Chenmeng; Li, Xiaoxiao; Ma, He; Wan, Chongqing; Li, Kai; Lin, Yuqing

来源：Biosensors and Bioelectronics, 2015, 65: 232-237.

DOI：10.1016/j.bios.2014.10.046

• Poly(diallyldimethyl ammonium chloride)
• Gold nanoparticles
• Graphene
• Aptamer
• Electrochemical
• Angiogenin

Herein, a label-free and highly sensitive electrochemical aptasensor for the detection of angiogenin was proposed based on a conformational change of aptamer and amplification by poly(diallyldimethyl ammonium chloride) (PDDA)-functionalized graphene/gold nanoparticles (AuNPs) composites-modified electrode. PDDA-functionalized graphene (P-GR) nanosheets as the building block in the self-assembly of GR nanosheets/AuNPs heterostructure enhanced the electrochemical detection performance. The electrochemical aptasensor has an extraordinarily sensitive response to angiogenin in a linear range from 0.1 pM to 5 nM with a detection limit of 0.064 pM. The developed sensor provides a promising strategy for the cancer diagnosis in medical application in the future.

• 出版日期2015-3-15
• 单位首都师范大学