Acetyl-coenzyme A synthetase 2 is a nuclear protein required for replicative longevity in Saccharomyces cerevisiae

作者：Falcon Alaric A; Chen Shaoping; Wood Michael S; Aris John P^*

来源：Molecular and Cellular Biochemistry, 2010, 333(1-2): 99-108.

DOI：10.1007/s11010-009-0209-z

• Acetyl-coenzyme A
• Aging
• Nucleus
• Saccharomyces cerevisiae

Acs2p is one of two acetyl-coenzyme A synthetases in Saccharomyces cerevisiae. We have prepared and characterized a monoclonal antibody specific for Acs2p and find that Acs2p is localized primarily to the nucleus, including the nucleolus, with a minor amount in the cytosol. We find that Acs2p is required for replicative longevity: an acs2a dagger strain has a reduced replicative life span compared to wild-type and acs1a dagger strains. Furthermore, replicatively aged acs2a dagger cells contain elevated levels of extrachromosomal rDNA circles, and silencing at the rDNA locus is impaired in an acs2a dagger strain. These findings indicate that Acs2p-mediated synthesis of acetyl-CoA in the nucleus functions to promote rDNA silencing and replicative longevity in yeast.

• 出版日期2010-1