NMR Structure of the S-Linked Glycopeptide Sublancin 168

作者：De Gonzalo Chantal V Garcia; Zhu Lingyang; Oman Trent J; van der Donk Wilfred A^*

来源：ACS Chemical Biology, 2014, 9(3): 796-801.

DOI：10.1021/cb4008106

Sublancin 168 is a member of a small group of glycosylated antimicrobial peptides known as glycocins. The solution structure of sublancin 168, a 37-amino-acid peptide produced by Bacillus subtilis 168, has been solved by nuclear magnetic resonance (NMR) spectroscopy. Sublancin comprises two a-helices and a well-defined interhelical loop. The two helices span residues 6-16 and 26-35, and the loop region encompasses residues 17-25. The 9-amino-acid loop region contains a beta-S-linked glucose moiety attached to Cys22. Hydrophobic interactions as well as hydrogen bonding are responsible for the well-structured loop region. The three-dimensional structure provides an explanation for the previously reported extraordinary high stability of sublancin 168.

• 出版日期2014-3