Association of CYP1A1, CYP1B1 and CYP17 gene polymorphisms and organochlorine pesticides with benign prostatic hyperplasia

作者：Kumar Vivek; Banerjee Basu Dev^*; Datta Sudip Kumar; Yadav Chandra Shekhar; Singh Satyender; Ahmed Rafat Sultana; Gupta Sanjay

来源：Chemosphere, 2014, 108: 40-45.

DOI：10.1016/chemosphere.2014.02.081

• Benign prostatic hyperplasia
• Organochlorine pesticides
• Cytochrome P450
• Polymorphism

It is well established that steroidal hormones (testosterone and estrogen) increase benign prostatic hyperplasia (BPH) risk. Cytochrome P450 (CYP) enzymes especially CYP1A1, CYP1B1 and CYP17 metabolize these hormones. Apart from that, several endocrine disrupting organochlorine pesticides (OCPs) are reported to mimic the activity of these steroidal hormones. Therefore, functional polymorphisms in these genes and exposure to such pesticides may increase BPH risk further. Our study included 100 newly diagnosed BPH subjects and 100 age-matched healthy male controls. CYP1A1, CYP1B1 and CYP17 polymorphisms were studied using PCR-RFLP and allele-specific PCR method. OCP levels in blood were analyzed by gas chromatography (GC). Levels of p,p%26apos;-DDE and endosulfan alpha were found to be significantly higher amongst BPH subjects as compared to controls (p-values = 0.001 and 0.03 respectively) and CYP17 polymorphism was observed to be significantly associated with BPH subjects as compared to controls (p-values = 0.03), indicating that these factors may be important risk factors for BPH. However, further studies are required before unequivocal conclusion.

• 出版日期2014-8