Distinct regulatory mechanisms governing embryonic versus adult adipocyte maturation

作者：Wang Qiong A; Tao Caroline; Jiang Lei; Shao Mengle; Ye Risheng; Zhu Yi; Gordillo Ruth; Ali Aktar; Lian Yun; Holland William L; Gupta Rana K; Scherer Philipp E^*

来源：Nature Cell Biology, 2015, 17(9): 1099-+.

DOI：10.1038/ncb3217

Pathological expansion of adipose tissue contributes to the metabolic syndrome. Distinct depots develop at various times under different physiological conditions. The transcriptional cascade mediating adipogenesis is established in vitro, and centres around a core program involving PPAR gamma and C/EBP alpha. We developed an inducible, adipocyte-specific knockout system to probe the requirement of key adipogenic transcription factors at various stages of adipogenesis in vivo. C/EBP alpha is essential for all white adipogenic conditions in the adult stage, such as adipose tissue regeneration, adipogenesis in muscle and unhealthy expansion of white adipose tissue during high-fat feeding or due to leptin deficiency. Surprisingly, terminal embryonic adipogenesis is fully C/EBP alpha independent, but does however depend on PPAR gamma; cold-induced beige adipogenesis is also C/EBP alpha independent. Moreover, C/EBP alpha is not vital for adipocyte survival in the adult stage. We reveal a surprising diversity of transcriptional signals required at different stages of adipogenesis in vivo.

• 出版日期2015-9