Maxillary sinus floor augmentation with injectable calcium phosphate cements: a pre-clinical study in sheep

作者：Hoekstra Jan Willem M; Klijn Reinoud J; Meijer Gert J; van den Beucken Jeroen J J P; Jansen John A^*

来源：Clinical Oral Implants Research, 2013, 24(2): 210-216.

DOI：10.1111/j.1600-0501.2012.02421.x

• animal experiments
• biomaterials
• bone regeneration
• bone substitutes
• guided tissue regeneration
• material sciences
• sinus floor elevation

Objectives The aim of this pre-clinical study was to evaluate the biological performance of two injectable calcium phosphate cement (CPC) composite materials containing poly(D,L-lactic-co-glycolic)acid (PLGA) microspheres with different properties in a maxillary sinus floor elevation model in sheep. Materials and methods PLGA microspheres were made of either low molecular weight (similar to 17 kDa) acid-terminated PLGA (PLGAL-AT) or high molecular weight (similar to 44kDa) end- capped PLGA (PLGAH-EC) and incorporated in CPC. Eight female Swifter sheep underwent a bilateral maxillary sinus floor elevation procedure via an extra-oral approach. All animals received both materials, alternately injected in the left and right sinus (split-mouth model) and a time point of 12 weeks was used. Analysis of biological performance was based on histology, histomorphometry, and evaluation of sequential fluorochrome labeling. Results Both types of CPC-PLGA composites showed biocompatibility and direct bone-cement contact. CPC-PLGAL-AT showed a significantly higher degradation distance compared to CPC-PLGAH-EC (1949 +/- 1295 mu m vs. 459 +/- 267 mu m; P = 0.0107). Further, CPC-PLGAL-AT showed significantly more bone in the region of interest (26.4 +/- 10.5% vs. 8.6 +/- 3.9% for PLGAH-EC; P = 0.0009) and significantly less remaining CPC material (61.2 +/- 17.7% vs. 81.9 +/- 10.9% for PLGAH-EC; P = 0.0192). Conclusions Both CPC-PLGAL-AT and CPC-PLGAH-EC demonstrated to be safe materials for sinus floor elevation procedures in a large animal model, presenting biocompatibility and direct bone contact. In view of material performance, CPC-PLGAL-AT showed significantly faster degradation and a significantly higher amount of newly formed bone compared to CPC-PLGA(H-EC).

• 出版日期2013-2