科研之友
微信
新浪微博
Facebook
分享链接
摘要
Garlic contains the organosulfur compound allicin which exhibits potent antifungal activity. Here we demonstrate the use of a highly simplified yeast chemical genetic screen to characterize its mode of action. By screening 24 validated yeast gene deletion "signature" strains for which hypersensitivity is characteristic for common antifungal modes of action, yeast lacking the high affinity Cu2+ transporter Ctrl was found to be hypersensitive to allicin. Focusing on transition metal related genes identified two more hypersensitive strains lacking the Cu2+ and Zn2+ transcription factors. Macl and Zapl. Hypersensitivity in these strains was reversed by the addition of Cu2+ and Zn2+ ions, respectively. The results suggest the antifungal activity of allicin is mediated through restricted Cu2+ and Zn2+ uptake or inhibition of Cu2+ and Zn2+ metalloproteins. As certain antimicrobial modes of action are much more common than others, the approach taken here provides a useful way to identify them early on.
- 出版日期2017-5-10
