Magic angle spinning NMR of viruses

作者：Quinn Caitlin M; Lu Manman; Suiter Christopher L; Hou Guangjin; Zhang Huilan; Polenova Tatyana^*

来源：Progress in Nuclear Magnetic Resonance Spectroscopy, 2015, 86-87: 21-40.

DOI：10.1016/j.pnmrs.2015.02.003

• Viruses
• Magic angle spinning
• HIV-AIDS
• CA capsid
• Bacteriophage

Viruses, relatively simple pathogens, are able to replicate in many living organisms and to adapt to various environments. Conventional atomic-resolution structural biology techniques, X-ray crystallography and solution NMR spectroscopy provided abundant information on the structures of individual proteins and nucleic acids comprising viruses; however, viral assemblies are not amenable to analysis by these techniques because of their large size, insolubility, and inherent lack of long-range order. In this article, we review the recent advances in magic angle spinning NMR spectroscopy that enabled atomic-resolution analysis of structure and dynamics of large viral systems and give examples of several exciting case studies.

• 出版日期2015-4