Antibiotic-dependent selection of E-coli clones with increased chaperone activity for highly efficient production of full-length soluble new delhi metallo-beta-lactamase

作者：Kozyr A V^*; Luneva N M; Khlyntseva A E; Shemyakin I G; Krasavtseva O N; Kolesnikov A V

来源：Molecular Genetics, Microbiology and Virology, 2013, 28(4): 147-155.

DOI：10.3103/S0891416813040046

• chaperone activity
• E. coli

New Delhi metallo-beta-lactamase (NDM-1) is a plasmid-borne enzyme conferring bacterial resistance to any known beta-lactam antibiotics. There is urgent need to develop efficient NDM-1 inhibitors and approaches for early diagnostics of NDM-1 that necessitates structural studies of the enzyme and analysis of the secretion pathways and localization of the protein. Recombinant full-length NDM-1 is produced in E. coli in active form and mostly accumulates in inclusion bodies. We developed a new system that uses antibiotic pressure to select E. coli producing increased quantities of the soluble NDM-1 and showed that the efficiency of production of the soluble NDM-1 depends on the level of chaperone activity in a bacterial cell.

• 出版日期2013-10