DM-GRASP/ALCAM/CD166 is required for cardiac morphogenesis and maintenance of cardiac identity in first heart field derived cells

作者：Gessert Susanne; Maurus Daniel; Brade Thornas; Walther Paul; Pandur Petra; Kuehl Michael

来源：Developmental Biology, 2008, 321(1): 150-161.

DOI：10.1016/j.ydbio.2008.06.013

• heart development
• Xenopus laevis
• DM-GRASP
• ALCAM

Vertebrate heart development requires specification of cardiac precursor cells, migration of cardiac progenitors as well as coordinated cell movements during looping and septation. DM-GRASP/ALCAM/CD166 is a member of the neuronal immunoglobulin domain superfamily of cell adhesion molecules and was recently suggested to be a target gene of non-canonical Wnt signalling. Loss of DM-GRASP function did not affect specification of cardiac progenitor cells. Later during development, expression of cardiac marker genes in the first heart field of Xenopus laevis such as Tbx20 and Tnlc was reduced, whereas expression of the second heart field marker genes lsl-1 and BMP-4 was unaffected. Furthermore, loss of DM-GRASP function resulted in defective cell adhesion and Cardiac morphogenesis. Additionally, expression of DM-GRASP can rescue the phenotype that results from the loss of non-canonical Wnt11-R signalling suggesting that DM-GRASP and non-canonical Writ Signalling are functionally coupled during cardiac development.

• 出版日期2008-9-1